Here we look at anti-CD3 clones and their recommended applications.Ĭlone CD3-12, specifically recognizes a conserved epitope within the cytoplasmic sequence of the human CD3ε chain. The epitope on the epsilon polypeptide is conserved among many species resulting in antibody clone, CD3-12, having a very broad species cross reactivity for the CD3 marker. Mouse T cell marker selection and expression guide
Human T cell marker selection and expression guide T cell receptor mini-review for detailed information on TCR activation, signaling, development and diversity.T cell overview including antibodies available to this important immune cell.Read our CD3 mini-review for detailed information on CD3 including its structure alongside that of the TCR, the genes involved in its expression, its function and the signal transduction pathways mediated by the CD3 complex.įor more information about T cells refer to the following resources available: As CD3 is present at all stages of T cell development, it is a highly effective T cell marker. CD3 is expressed by a high-percentage of circulating peripheral T cells forming a complex with the T cell receptor (TCR).
Each of these chains contain immunoreceptor tyrosine activation motifs (ITAMS) which are required for initiation of signaling cascades, as they recruit protein tyrosine kinases, signaling intermediates and adapter molecules. It is a multimeric protein composed of four distinct polypeptide chains (ε, γ, δ, ζ). CD3 is a member of the immunoglobulin superfamily and acts as a mediator of signal transduction.
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